Abstract

INFLUENCE OF AN ETHYL ACETATE EXTRACT OF HOLARRHENA FLORIBUNDA (G. DON) DURAND AND SCHINZ (APOCYNACEAE) ON BLOOD CELLS IN NORMAL RATS, PLANT USED IN TRADITIONAL MEDICINE FOR TREATING DIABETES IN CÔTE D’IVOIRE

Kouassi Kousso Brigitte, *Gnangoran Boua Narcisse, Adjambri Adia Euserbe, Koné Mama and Yapo Angoué Paul

ABSTRACT

Holarrhena floribunda is a medicinal plant used in traditional to treat various pathologies in Côte d'Ivoire including diabetes. The present study was aimed to evaluate the safety of an ethyl acetate extract of the leaves of Holarrhena floribunda. Forty rats were used to study the haematological parameters effects. This extract was administered orally daily for 28 days to rats at 1000 mg/kg body weight. The control group received distilled water. A blood sample is taken once a week to determine the effect of the extract on blood haematological parameters. The results of the study showed that ethyl acetate extract was not changed structural abnormalities of erythrocytes, leukocytes and thrombocytes in rats treated compared to the control group. In addition, blood smear showed no abnormalities in size, shape and staining at the level of erythrocytes at a dose of 1000 mg/kg body weight for 28 days of treatment. The study of the reversibility of toxic effects after discontinuation of treatment showed that all haematological parameters studied were not significantly affected. This study revealed that ethyl acetate extract from the leaves of Holarrhena floribunda is non-toxic to blood cells for 28 days of treatment.

Keywords: Holarrhena floribunda, safety, haematological parameters, rat.