Abstract

EXTRACTION AND EVALUATION OF CARDIAC DEPRESSANT AND CYTOTOXIC ACTIVITIES OF BACOPA MONNIERI L.

K. N. S. Karthik*

ABSTRACT

Bacopa monnieri is a well known herb for its use in enhancing memory and in treatment of epileptic disorders ever since from ancient times. While effects of Hydro methanolic extracts of Bacopa monnieri on isolated heart has not been reported yet, so in this present study we aim to investigate effect of 75% hydro methanolic extract of Bacopa monnieri on isolated frog heart and cytotoxic activity by using Human MCF-7 cell line. The extract produced significant results by dramatically decreasing the heart rate in a tachycardia induced heart. It shows decrease in the heart rate with an increase in the concentration of dose, showing a negative inotropic and chronotropic effects at 50μg/ml and 75μg/ml. where at 25μg/ml it showed a significant decrease in the rate of contraction but no change in the heart rate. 1 μg/ml Adrenaline was used to induce the Tachycardia. The in-vitro cytotoxic study was carried out by following the determination of cell viability by MTT assay on MCF-7 cell line. It produced a significant result with an IC50 median value of 370±0.0μg/ml. The current study reveals that the Hydro methanolic extract of Bacopa monnieri has a significant cardiac depressant activity and also the cytotoxic activity in a dose dependent manner.

Keywords: Methanolic extract, Bacopa monnieri, cardiac depressant, MCF, MIT assays, cytotoxic.