Abstract

SYNTHESIS, SPECTRAL CHARACTERIZATION, DNA INTERACTION AND ANTICANCER EVALUATION OF TRANSITION METAL COMPLEXES OF HYDROXAMIC ACID DERIVATIVE

Divya S. Hegde and Kalagouda B. Gudasi*

ABSTRACT

A novel tetradentate chelating ligand, N'-(1-(hydroxyamino)-1-oxopropan-2-ylidene)pyrazine-2-carbohydrazide and its Fe(III), Co(II), Ni(II), Cu(II) and Zn(II) complexes were synthesized and characterized by elemental analyses, spectral (vibrational, electronic, 1H NMR,13C NMR and Mass) and thermal studies. The interaction of ligand and complexes with calf-thymus DNA (CT-DNA) has been extensively studied using absorption, emission, viscosity and thermal denaturation studies with E. coli DNA. The DNA cleavage ability of ligand and metal complexes was tested using plasmid pBR322 DNA by gel electrophoresis method. The compounds were evaluated for their in vitro antiproliferative activity against human cancer cells of different origin such as MCF-7, Mia-Pa-Ca-2 and DU-145 by using SRB(sulforhodamineB) assay. The copper and iron complex have shown better anticancer activity as compared to other compounds under study.

Keywords: Hydroxamic Acid; Ethyl pyruvate; Transition Metal Complexes; DNA Binding and Cleavage Studies; Anticancer studies.