Abstract

SIMULTANEOUS ESTIMATION OF ACEBROPHYLLINE, MONTELUKAST AND LEVOCETRIZINE DIHYDROCHLORIDE IN MARKETED FORMULATION BY UV-SPECTROSCOPY

Mohini Mittal, Yogesh Upadhyay, Durgadas Anghore and Ravindra K. Rawal*

ABSTRACT

We have developed methods for simultaneous estimation of Acebrophylline (ABP), Montelukast (MLK) and Levocetirizine Dihydrochloride (LCZ) in pure and marketed formulation by UV-Spectroscopy. Methods are simple, accurate, rapid, precise, economical and reliable. For the simultaneous estimation of this combination, Vierdot’s method (Method I) and First Derivative method (Method II) were performed. Methanol was used as diluents. Absorbance maxima for ABP, MLK and LCZ were found to be 250.14, 284.79 and 231.27 nm, respectively for Method I. For Method II absorbance maxima for ABP, MLK and LCZ were found to be 275.15, 296.26 and 246.61 nm, respectively. Both methods obeys Beer’s law in the concentration range of 6-18 μg/mL for ABP (R2 = 0.9994 for Method I; R2 = 0.9996
for Method II) and MLK (R2 = 0.9982 for Method I; R2 = 0.998 for Method II) and 3-12μg/mL for LCZ (R2 = 0.999 for Method I and Method II). LOD and LOQ of Method I for ABP were found to be 0.29 μg/mL and 0.6 μg/mL, for MLK 0.25 μg/mL and 0.75 μg/mL and for LCZ 0.46 μg/mL and 1.42 μg/mL, respectively whereas for Method II LOD and LOQ for ABP were found to be 0.25 μg/mL and 0.8 μg/mL, for MLK 0.28 μg/mL and 0.9 μg/mL and for LCZ 0.52 μg/mL and 1.6 μg/mL, respectively. Percentage purity of ABP, MLK and LCZ in assay of marketed formulation (Coekastle-A) was found to be 100%, 101% and 99%, respectively. The methods showed good reproducibility and recovery with % RSD ˂ 2%.

Keywords: UV-Visible spectroscopy, Acbrophylline, Montelokast sodium, Levocetirizine dihydrochloride.