Abstract

SPECTROPHOTOMETRIC DETERMINATION OF DRUGS BY USING N-BROMOSUCCINAMIDE AND RHODAMINE-B DYE COUPLE

G. Pranitha and G. Venkateshwarlu*

ABSTRACT

Simple, sensitive and accurate methods are developed for the spectrophotometric determination of five drugs, viz., Amoxicillin, Bendamustine, Ceftriaxone Sodium, Diclofenac Sodium and Quetiapine Fumerate based on their reactivity towards N-Bromosuccinamide(NBS) . The method involves addition of a known excess of N-bromosuccinimde to drugs in acidic medium (1M HCl) and the residual amount of oxidant (NBS) is estimated with Rhodamine-B dye. The absorbance was measured at 557 nm. These methods have been applied for the determination of above drugs in their pure form as well as in tablet formulations. The method has been validated in terms of guidelines of ICH.

Keywords: Spectrophotometry, NBS, Rhodamine-B, drugs, Quantification and Validation.