Abstract

PHYTOCHEMICAL ANALYSIS OF TRIGONELLA FOENUM GRAECUM AND ITS ANTIBACTERIAL ACTIVITY AGAINST STAPHYLOCOCCUS AUREUS

Dr. Renu Mishra*, Shikha Mandloi, Nishi Yadav and Jyoti Choithani

ABSTRACT

Disease causing bacteria have always been considered a major cause of morbidity and mortality in humans. The appearance of multidrug resistant microorganism have paved the way to search for new antimicrobial drugs. Plants are good source of compounds which are of great significance in therapeutic treatment and help to cure the problem of multidrugs resistant organism. In the present study Staphylococcus aureus bacteria was isolated from the sputum of Mycobacterium negative patients. Seeds of plant Trigonella foenum graecum (methi) were taken for herbal drug potential studies. Phytochemical analysis of seeds was done by Soxhlet apparatus in acetone and methanol solvents. Results showed that, seeds have flavonoids, alkaloids, phenolic compound and carbohydrate present in both the extracts. The antibacterial activity of Trigonella foecum graecum was evaluated against human pathogenic bacteria Staphylococcus aureus by agar diffusion method. Methanol and acetone seed extracts of Trigonella were investigated for in vitro antibacterial activity. It has been observed that methanol and acetone extract of Trigonella were effective against Staphylococcus aureus at 100 mg/ml and 200mg/ml. From this study it is concluded that methi seeds are effective in the inhibition of Staphylococcus aureus growth in vitro conditions.

Keywords: Bacteria, Staphylococcus aureus, Trigonella foenum graecum, flavonoids, alkaloids, Sputum.