Abstract

STUDYING THE CLONING AND EXPRESSION OF ?-GLUCOSIDASE USING YAKULT AND STRAWBERRY PROBIOTICS AS POTENTIAL SOURCE OF BIFIDBACTERIUMBREVE

P. Singh*, A.N. Singh and Madhulika Singh

ABSTRACT

Bifidobacteriumbreve is important for food industries since it is known to be a great source of β-glucosidase which helps in lactose digestion. In the present study the samples of Strawberry and Yakult were collected for the isolation of bacterial species using spreading and streaking techniques. The isolated bacterial species were identified by performing biochemical tests. Genomic DNA from B.breve was cleaved with the restriction enzyme SmaI andβ-glucosidase geneisligated to pBR322 for transformation into Escherichia coliDH5 α. β-glucosidase gene was functionally expressed in E.coli DH5 α; the transformants were able to grow on ampicillin.β-glucosidase positive clone was demonstrated in the results by presence of its colonies on a medium supplemented with ampicillin.

Keywords: ?-glucosidase, Probiotics, Bifidobacteriabreve and Escherichia coli DH5 ?.