Abstract

BIOCHEMICAL ALTERATION IN SELECTED BODY TISSUES OF FRESH WATER PRAWNS, MACROBRACHIUM ROSENBERGII EXPOSED TO MONOCROTOPHOS

Dr. J. Jayachitra*, P. Ilavarasi and R. Subashini

ABSTRACT

Objective: The stydy aimed at assessing the biochemical alteration in selected body tissues of fresh water prawns, Macrobrachium Rosenbergii exposed to monocrotophos. Methods: 200 Female freshwater prawns, macrobrachium rosenbergii were collected from the kollidam near Annaikkarai. One group was considered as control and another group as the experimental group was exposed to acute (short term) treatments the acute treatment consisted of exposure to 9.5ppm (1/2 of 96h lc50) and chronic treatment consisted of exposure to 1.9 ppm (MCP)twenty test prwns in each set of both controll and experimental groups were maintained simutaneously. The medium water and monocrotophos (MCP) Consentration were renewed daily. the prwns were sacrificed at interval of 24h in the acute and at intervls of 7.14 and 21days in the chronic treatment groups for biochemical studies. In order to evaluate the total protein content and total lipid, the selected tissues namely. The gills, hepatopancreas and muscle of acute and chronic exposed m. resenbergii. At the end exposure peried (24-96h; 7-21 days) the prawns of both controll and groups were anaethetized 1:3000 MS 222(tricane methene sulfonate, Sandoz) for 2minutes and the test tissues (Gills, hepatopancreas and muscle) were quickly dissected out Weighed to the nearest milligram.

Keywords: Macrobrachium Rosenbergii, Biochemical parameters and monocrotophos.