Abstract

IN- VITRO STUDIES OF A VALUABLE MEDICINAL PLANTARTEMISIA VULGARIS L. (MUGWORT)

*Yatoo Ghulam Mohiuddin and Nathar Varsha Nitin

ABSTRACT

The objective of the present investigation was to develop an in vitro protocol for mass propagation of Artemisia vulgaris L. This will help in its conservation as well as for its genetic transformation system. In vitro seed germination started in MS media supplemented with 8% (v/v) filtered sterilized coconut water after 13 days of inoculation. Highest callogenic frequency i.e., 85 ± 0.46% was observed from shoot tip explant at 0.75 mg/l kinetin. Maximum number of shoots i.e., 16 ± 1.19 and maximum shoot length (cm) i.e., 6.3 ± 0.14 was observed at kinetin 1 mg/l. Maximum number of roots i.e., 15 ± 1. 03 and maximum root length (cm) i.e., 7.2 ± 1.15 was observed at IAA 1 mg/l and 0.5 mg/l NAA respectively.

Keywords: Asteraceae, coconut water, conservation, in vitro and shoot tip explant.