Abstract

A VALIDATED STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF NETUPITANT AND PALENOSERTON IN PHARMACEUTICAL FORMULATIONS

Mangesh Harole*, R. N. Patil and Deepak Gaware, Govind Suryawanshi and Kalyan Pise

ABSTRACT

The proposed study, a new stability–indicating RP- HPLC method has been developed for estimation of netupitant and palonoserton in bulk and pharmaceutical dosage form. The present method was a sensitive, precise and accurate RP-HPLC method for analysis of netupitant and palonoserton. To optimize mobile phase, various combination of buffer and organic solvent were used on Thermo BDS C 18 (150 mm x 4.6 mm, 5μ) column. Then mobile phase containing mixture of Phosphate buffer and acetonitrile in ratio of 40:60% v/v was selected at flow rate 1.0 ml/min for developing method and peaks with good shape and resolution were found short retention time, baseline stability and minimum noise. The retention time of netupitant and palonoserton was found to be 2.8 min and 2.3 min respectively. Quantitative linearity was obeyed in the range 0-450 and 0-.75μg/ml of netupitant and palonoserton respectively. The limit of detection and limit of quantitation were found to be 0.11 μg/ml and 0.34 μg/ml (netupitant); 0.16μg/ml and 0.48μg/ml (palonoserton) respectively, which indicate sensitivity of the method. The high percentage of recovery indicates the proposed method is highly accurate. No interfering peaks were found in the chromatogram indicating that excipients used in capsules formulation didn’t interfere with the estimation of the drugs by the proposed HPLC method.

Keywords: chromatography, Netupitant and Palonoserton.