Abstract

PROTECTIVE EFFECT OF BIOSURFACTANT PRODUCED BY LEUCONOSTOC MESENTEROIDES SSP.CREMORIS AGAINST SALMONELLA TYPHI IN MICE

Adnan Yaas Khudair*, Suhair S. Majeed, Al Maamon H. Abed, Enaam H. Batah, Roaa F. Mansour and Rayim S. Abbood

ABSTRACT

The protective effect of the biosurfactant produced from Leuconostoc mesenteroides ssp.cremoris against the pathogenic Bacteria Salmonella typhi was studied in vitro and in vivo. The inhibitory effect of the biosurfactant was tested with the Well diffusion method, the biosurfactant showed a clear inhibitory effect against the pathogenic bacteria. A group of mice were administered orally with (0.25 ml) of the biosurfactant for 10 days, then they given (0.2 ml) of the Salmonella typhi living cells ( cell\ml) orally, The control group were given 0.25 ml of PBS only. The mice were killed, and asamples of the liver for all groups were taken and histopathological changes were recorded. The results shows histological changes only in the Liver when given the mice group with S.typhi, the microscopic examination of the Liver tissue shows a decay and local area of necrosis with inflammatory cells.

Keywords: Leuconostoc mesenteroides ssp.cremoris, Salmonella typhi.