Abstract

SIMULTANEOUS ESTIMATION OF INDAPAMIDE AND PERINDPRIL IN TABLETS BY RP-HPLC METHOD

Rama Prasad Padhy, K. Srinivas, Subas Chandra Dinda

ABSTRACT

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Indapamide and perindopril in tablets. A column having 150 x 4.6 mm, 3μ i.d. in gradient mode with mobile phase containing Ammonium Dihyrogen Phosphate: Acetonitrile (60:40) was used. The flow rate was 1.0 ml/min and effluent was monitored at 230 nm. The retention time (min) and linearity range (ppm) for indapamide and perindpril were (4.32, 2.31) and (2-12, 10-60), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of indapamide and perindopril in tablets.

Keywords: Indapamide, Perindopril Erbumine, RP-HPLC, simultaneous determination, tablets.