Abstract

NEW TECHNIQUE FOR EXTRACTION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM IRAQI VITIS-VINIFERA LEAVES

Alaa Kamal Jabbar Alhamd*, Falah Sumoom, Kadhim Hashim Zuein,
Ali Murtadha Hassin, Ali Abdul Rasool

ABSTRACT

Natural products from medicinal plants either as pure or as standardized extract provide unlimited opportunities for new drug leads because of the unmatched availability of chemical diversity. The aqueous extracts of Vitis-Vinifera Leaves were prepared by taking the fresh leaves from Iraqi Vitis-Vinifera tree and washed under running tap water then choped by knife to small pieces. Air dried at room temperature(25oC) and dark place to avoid the effectivenees of high temperature and sun light . The chopped leaves were collected and ground to fine powder and stored in airtight dark bottles. For aqueous extract, 50 g of air-dried powder was added to 200ml distilled water and gently heated on slow rate rising temperature reached to 470C for 10 hours with continuous stirring then leave on punch to cool. The mixture was collected in airtight ember color flask and filtered through filter paper ( wattman-5). The filtration process was repeated at least three times. The supernatant was collected to ember color flask. The final (150 ml) was concentrated to one-tenth of the original volume by heating gently to 40-45 0C using rotary evaporator. The aqueous extract was analyzed the phenolic compounds in crude extract by using Ultra Violet-Visible "UV- VIS" " spectrophotometer and Fourier Transform Infrared Spectroscopy"FTIR". Aqueous extract was evaluated for antibacterial activity against some types of Gram-positive and Gram-negative bacteria. The invitro antibacterial activity was performed by agar well diffusion method and the minimum inhibitory concentration (MIC) was determined by micro- titration technique. The results were indicated that the aqueous extract was inhibited some of Gram-positive bacteria as well as two Gram-negative bacteria.

Keywords: Vitis-Vinifera Leaves, Polyphenolic compounds, Antibecteral agent.