Abstract

BROCCOLI SULFORAPHANE: AN INSIGHT INTO THE ANALYTICAL ASPECT AND ULTRAVIOLET SPECTROSCOPIC METHOD DEVELOPMENT AND VALIDATION

Kriti Soni and Dr. Kanchan Kohli*

ABSTRACT

Sulforaphane is an isothiocyanate present in broccoli which is cleaved out of glucoraphanin as a result of activity of enzyme myrosinase. It is insoluble in water but shows good solubility in organic solvents. In addition to this the drug is highly prone to degradation subjected to thermal and basic stresses. Inspite of these it has been found to be highly active as an anticancer molecule which works at a very low dose and shows almost no toxic effect. The present paper highlights the extraction of sulforaphane from broccoli, its structural elucidation by simulation studies and development and validation of its ultraviolet spectroscopic method of analysis. The paper also highlights various important analytical methods developed by other researchers such as HPLC, GC-MS, LC-MS, FTIR, NMR and Mass spectroscopic analysis. These methods have been discussed here in so as to provide readers with an insight into the analytical methodologies of these compound. The development of these methods by authors will be discussed in subsequent papers.

Keywords: Sulforaphane, Glucoraphanin, HPLC, LC-MS, Ultraviolet spectroscopy, Validation.