Abstract

PUPICIDAL ACTIVITY AND MORPHOLOGICAL DEFORMITIES OF JUSTICIA ADHATODA LEAF EXTRACTS AGAINST FILARIAL VECTOR, CULEX QUINQUEFASCIATUS AND DENGUE VECTOR, AEDES AEGYPTI

Jayapriya Gopal* and Gricilda Shoba F

ABSTRACT

The present study deals with the investigation of pupicidal activities of petroleum ether, chloroform, ethyl acetate and methanol leaf extract of Justicia adhatoda against Culex quinquefasciatus and Aedes aegypti. 25 pupae of C. quinquefasciatus and A. aegypti was exposed to various concentrations and was assayed in the laboratory by using the protocol of WHO (1996); the assay was carried out for 24h and 48h; LC50 and LC90 values of the J. adhatoda leaf extract was determined by Probit analysis. The LC50 and LC90 values of methanol leaf extract of J. adhatoda against pupal stages of C. quinquefasciatus were 153.73, 97.04 and 427.40, 410.36 ppm and A. aegypti were 161.30, 106.79 and 425.20, 431.53 ppm respectively at 24h and 48h. Maximum pupicidal activity was observed in the methanol extract. No mortality was observed in control. Among four solvent tested the methanol and petroleum ether extracts were most effective against C. quinquefasciatus and A. aegypti. From the results it can be concluded the crude extract of J. adhatoda was an excellent potential for controlling C. quinquefasciatus and A. aegypti mosquitoes.

Keywords: Pupicidal, Justicia adhatoda, Culex quinquefasciatus, Aedes aegypti, Morphological Deformities.