Abstract

CURRENT PERSPECTIVE ON SOLVENT FREE ANALYSIS IN PHARMACEUTICAL INDUSTRY

Dr. Jignesh S. Shah*, Priti V. Patel and Dr. Dilip G. Maheshwari

ABSTRACT

Sample preparation is an essential step in Pharmaceutical analysis, greatly influencing the consistency and reliability of produced time and cost of analysis.Classical sample preparation methods are time consuming, tough, or limited to automate. Solid-Phase Microextraction (SPME) is a very simple and proficient, solventless sample preparation method in which extraction, concentration, (derivitisation) and transfer to the chromatograph are integrated into one step and device, which simplifying the sample preparation procedure. Solid-Phase Microextraction (SPME) was developed in 1990 using a fused silica fiber coated on the outside with appropriate stationary phase in which analyte in the sample is directly extracted to the fiber coating. Mainly two types of fiber techniques can be used to extract analyte Headspace (HS-SPME), where the fibers are exposed to the vapour phase above a gas, and direct immersion (DI-SPME), where the fiber is directly immersed in the samples. More innovative in-tube SPME recently has been established, which uses open tubular fused-silica capillary column instead of SPME fiber. SPME in combination with HPLC, GC, LC-MS, GC-MS, Electrospray ionization mass spectroscopy, Capillary electrophoresis is getting a wide adequacy as an analytical technique. These have in ordinate benefits over the classical sampling techniques, which are time consuming and have need of more samples and solvents.

Keywords: Microextraction, Pharmaceutical industry, Solid phase Microextraction, Silica fibers.