Abstract

BIO-ANALYTICAL METHOD DEVELOPEMENT AND VALIDATION OF LACIDIPINE IN HUMAN PLASMA USING AMILODIPINE AS INTERNAL STANDARD BY LC/MS/MS

K. Kanaka Parvathi* and Hanumanthachar joshi

ABSTRACT

The present manuscript describes bio-analytical method developement and validation of lacidipine in human plasma using amilodipine as internal standard by liquid chromatography and tandem mass spectrometry method lc/ms/ms. The column employed in this determination was Agilent, Eclipse Zorbax XDB phenyl (4.6x75, 5μ), mobile phase comprising 5M ammonium acetate buffer: isopropnol in the ratio of 70:30v/v. the injection volume were 20 μL-1 the retention time was about 1.3 for lacidipine and 0.95 minutes for Amilodipine of a total run time of 2.5 minutes, linearity of the method was linear over the range of 0.2052 ng/mL to 12.5026 ng/mL using Amilodipine as internal standard.

Keywords: Lacidipine, Amilodipine.