Abstract

THE EFFECT OF MULBERRY (MORUS SP.) TEA SUPPLEMENT ON ACETAMINOPHEN INDUCED RENAL FAILURE IN RATS

Noman D Salih*, Nur Shukriyah M Hazir, Mohamad H Abo Hamid

ABSTRACT

Mulberry (Morus sp.) is a rich source of antioxidants and phenolic compounds. Its leaves possess many bioactive compounds, such as deoxymycine and anthocyanin, and high levels of calcium, magnesium, potassium, sulfate, zinc and vitamins. Regular consumption of mulberries thought to provide potential against cancer, aging, neurological diseases, inflammation, diabetes and bacterial infections. This study has investigated the effect of mulberry tea supplement on the cellular damage of kidney (nephrotoxicity) induced by supratherapeutic acetaminophen administration. Three groups of rats (6 animals each) used, treated group (rats were fed with mulberry tea extract supplemented), positive control and negative groups (rats were fed with normal pellet diet). After 7 days animals of treated group and positive control injected with acetaminophen, 1000 mg/kg bw dissolved in 5 ml of normal saline, while rats of negative control injected with 5 ml of normal saline only. Kidneys removed, processed, sectioned, and stained for histological examination. Glomerular morphometric analysis (GMA) has performed to evaluate kidney glomerular morphology. Food intake monitored along the experiment, biological value of diet intake assessed, by its effect on body weight gain (BWG) and feed efficiency ratio (FER). Aqueous extraction of mulberry tea has prepared and total phenolic compounds in the sample quantified. Results showed mulberry tea extract supplement did help to maintain the kidney closer to normal and serve to protect it from severe damage due to nephrotoxicity, compared to animals that didn’t receive the supplement. Mulberry extract supplement could serve as a candidate for developing safe, complaisance and promising nutraceutical product for the management of nephrotoxicity.

Keywords: Mulberry extracts supplement, kidney failure, acetaminophen, nephrotoxicity