Abstract

HPTLC FINGERPRINTS OF ESSENTIAL OILS IN OROXYLUM INDICUM (L.) VENT

Saraf Aparna*, K. Srilatha Srinivas

ABSTRACT

The study was aimed to develop the HPTLC fingerprints of Essential oils for leaf , stem and roots of the medicinally important plant Oroxylum indicum (L.) Vent collected from two different geographical regions of India viz., Western Ghats and North East. Preliminary phytochemical screening was done followed by extraction of Essential oils and then by HPTLC studies. Toluene : Ethyl acetate : Diethyl amine ( 7:2:1) was used as mobile phase along with 5% vanillin in H2SO4 as derivatizing agent for the separation of Essential oils. A good seperation of Essential oils was observed at 366 nm after derivatisation. The study revealed diverse forms of Essential oils in large number in the root, stem and leaf of Oroxylum indicum (L.) Vent. from both the regions. It can be concluded that HPTLC fingerprint analysis of root, stem and leaf extract of Oroxylum indicum (L.) Vent can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker. These separated Essential oils in addition of being developed directly as drugs can also served as prototype drug molecules known as “Lead Compounds” and as pharmacological probes to help better understand biochemical and physiological mechanisms.

Keywords: Oroxylum indicum (L.) Vent, Essential oils, HPTLC fingerprint.