Abstract

EVALUATION OF ANTI-DIABETIC ACTIVITY OF URSOLIC ACID ACETATE ISOLATED FROM COLEUS VETTIVEROIDES (JACOB) IN STREPTOZOTOCIN- INDUCED DIABETIC RATS

*G. Gopalakrishnan , C.K.Dhanapal

ABSTRACT

Objective: The aim of the study was to evaluate the anti-diabetic activity of Ursolic acid acetate isolated from methanolic extract of Coleus vettiveroides (Jacob) in streptozotocin induced diabetic rats. Methods: Streptozotocin was used to induce diabetes mellitus. The anti-diabetic potential was assessed by determining oral glucose tolerance, fasting blood glucose level, urine glucose estimation, changes in body weight, Liver functions (SGPT and ALP), kidney functions (Urea and Creatinine), Pancreatic functions (Liver Glycogen and Serum Amylase) and Serum parameters (Total Bilirubin, Total Protein, Total Cholesterol, Triglycerides) and histopathological studies was done for the control and experimental rats. Results: Ursolic acid acetate isolated from Methanolic extract of Coleus vettiveroides (Jacob) was administered to normal and experimental diabetic rats for 15 days.Significant reduction in fasting blood glucose levels was observed in the Ursolic acid acetate is treated diabetic animals from day 7 onwards. In oral glucose tolerance test, reduction in fasting blood glucose a level was noted after 60 min of extract administration. After 15 days of treatment with extracts the maximum reduction in fasting blood glucose level was observed in diabetic rats treated with Ursolic acid acetate isolated from methanolic extract of Coleus vettiveroides (Jacob) (50mg/kg /kg body wt) and the loss of body weight was controlled in treated rats as compared to diabetic control. The extract treatment also showed a significant decrease in urine sugar level.Streptozotocin treatment leads to elevated levels of Serum Amylase triglycerides, Total Cholesterol, SGPT, ALP, Bilirubin, Blood Urea and Creatinine and decreased the levels of Liver Glycogen and Total Protein. However, treatment with Ursolic acid acetate isolated from methanolic extract of Coleus vettiveroides (Jacob) significantly reversed the above changes compared to the control group as observed in the streptozotocin-induced rats.Microscopically examined pancreas section of rats treated with Ursolic acid acetate showed normal architecture of pancreas. Conclusion: The results demonstrate that Ursolic acid acetate isolated from methanolic extract of Coleus Vettiveroides (Jacob) possesses significant anti-diabetic activity.

Keywords: Coleus Vettiveroides Jacob, Streptozotocin, Serum parameters,Ursolic acid acetate,Isolated compound.