Abstract

STABILITY-INDICATING SPECTROPHOTOMETRIC METHOD OF ACYCLOVIR IN BULK AND PHARMACEUTICAL DOSAGE FORM

Khokale Anil. S* and Patil Pallavi. M

ABSTRACT

To develop simple, accurate, precise and cost effective UV-VIS Spectrophotometric method for the estimation of Acyclovir, an antiHIV drug, in bulk and pharmaceutical dosage form. The solvent used was 0.1N Sodium Hydroxide and the λmax or the absorption maxima of the drug was found to be 260nm. Acyclovir was subjected to stress degradation under different conditions recommended by ICH. The sample so generated were used for degradation studies using the the developed method. A linear response was observed in the range of 1 - 10µg/ml with a regression coefficient of 0.999. The method was then validated for different parameters as per the ICH (International Conference on Harmonization) guidelines. The stress degradation studies showed that Acyclovir undergoes degradation in acidic and alkaline conditions whereas it is relatively stable when exposed to dry heat, oxidation and photolytic degradation.This method can be used for the determination of Acyclovir in quality control of formulation without interference of the excipients.

Keywords: Acyclovir, Degra dation, Stability- Indicating, UV-Vis Spectro photometry, Validation.