Abstract

IN VITRO EVALUATION OF ANTI-OXIDANT AND ANTI- PROLIFERATIVE ACTIVITY IN CRUDE EXTRACTS OF ARGYREIA NERVOSA ROOTS.

Bhoomi B. Joshi, Piyush chudasama Jagdish Patel and Kinnari N Mistry*

ABSTRACT

The anti-oxidative and anti-proliferative activities of various extracts of the roots of Argyreia nervosa were studied using in vitro standard assays viz. DPPH radical scavenging, ABTS radical scavenging, lipid peroxidation, iron chelating activity, superoxide scavenging Nitric oxide scavenging, total antioxidant capacity, total phenol and total flavanoids. The aqueous, methanolic and ethanolic extracts of A. nervosa (100, 200, 300, 400 and 500 μg/ml) exhibited concentration-dependent antioxidant activity. The aqueous methanolic extract successfully inhibited the proliferation of cancerous cells (HeLa and HEK293T cell lines) in 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). Thus, we show the potential concentration-dependant antioxidant and antiproliferative activities A. nervosa root extract.

Keywords: Argyreia nervosa, invitro, antioxidant activity, antiproliferative activity.