Abstract

INVITRO EVALUATION OF ANTIBACTERIAL ACTIVITY OF CALENDULA OFFICINALIS AGAINST MDR PATHOGENS

Amit Pandey*, Ekta Chandel

ABSTRACT

The present study was carried out by evolution of calendula officinalis against MDR pathogens. In process to preparation of plant extract, organic solvent (methanol 80%) and aqueous solvent (hot water extraction) had been used. Calendula’s leaves, roots, flower, branches, and stem had been used to study the antimicrobial activity. In calendula the best result had been observed in methanolic extract of roots with zone of inhibition of 39mm in case of E.coli as compare to other organic and aqueous extract of the sample. In case of MDR isolates the best result were seen when mixed extract had been used for AST (10μl of each methanolic extract) by agar well diffusion method with formation of ZOI of 20mm. The least concentration was found in methanolic extract of roots 0.01μg/ml. On Phytochemical screening of methanolic extract of calendula -tannin, cardiac glycoside and terpenoids were present in the form of secondary metabolites.

Keywords: Phytochemical analysis, MIC, antimicrobial sensitivity test, secondary metabolites, calendula officinalis, MDR pathogens.