Abstract

IN VITRO: PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTIOXIDANT POTENTIAL OF VITEX NEGUNDO. LINN OF SYNTHESIZED SILVER NANOPARTICLES

Amudha Murugan and Krishna kumari Shanmugasundaram *

ABSTRACT

The environmental friendly synthesis of nanoparticles process is a revolutionary step in the field of nanotechnology. In this study, the biosynthesis of silver nanoparticles was carried out using Vitex negundo leaf extract as reducing agent. UV–visible spectroscopy was used for quantification of silver nanoparticle synthesis. The synthesized silver nanoparticles were characterized with Scanning electron microscopy (SEM),Transmission electron microscope (TEM), Energy dispersive x - ray analysis (EDX), X-ray Diffraction (XRD) and Fourier transform Infrared Spectroscopy (FTIR). Phytochemicals are screened in order to predict the secondary metabolites in the extract. The in vitro antioxidant properties of the biosynthesized silver nanoparticles have been evaluated and these nanoparticles were found to have higher antioxidant capacity and thus it can be used as potential radical scavenger against deleterious damages caused by the free radicals.

Keywords: Vitex negundo, Silver nanoparticles, phytochemicals, free radicals, oxidative stress.