Abstract

PRELIMINARY PHYTOCHEMICAL STUDY, INVITRO ANTI-MICROBIAL AND ANTI-OXIDANT ACTIVITY OF ALCOHOLIC EXTRACT OF MUCUNA PRURIENS LEAVES

Ramya Kuber B*, Swapna B , Tejaswi M, Triveni M

ABSTRACT

The antioxidant activity of alcoholic extract of leaves of Mucuna pruriens (MP) of family Fabaceae was studied using three invitro assays. The antioxidant activity of alcoholic extract of MP was evaluated by scavenging of DPPH, Lipid Peroxidation, Nitric oxide Scavenging activity. The concentration of alcoholic extract were (3-800 ug/ml). The MP showed maximum scavenging activity at concentration of 800 μg/ml. The percent inhibition of the scavenging of the DPPH was found to be 80%, Lipid Peroxidation was 86% and nitric oxide scavenging activity was found to be 69%. The antibacterial activity of the alcoholic extract of Mucuna pruriens leave was carried out and it was determined by the cup plate and disc plate methods against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis. Streptomycin was used as a positive control. The zone of inhibition of alcoholic extract of MP against various microorganisms was measured and compared with standard control, MP showed maximum activity at the concentration of 200 mg/kg against all the bacterial strains, Maximum antibacterial activity against Bacillus subtilis and Pseudomonas aeruginosa and exhibited moderate antibacterial activity against Staphylococcus aureus and Escherichia coli. on the basis of the results obtained in the present study, the antioxidant activity of MP and antimicrobial activity may be due to presence of the active principle present in MP leaves. These invitro assays indicate that this plant extract is a significant source of natural antioxidants, which might be helpful in preventing the progress of various oxidative stresses. The finding justify the therapeutic application of the plant in the indigenous system of medicine, augmenting its therapeutic value. Preliminary Phytochemical analysis showed the presence of flavonoids, alkaloids, tannins, amino acids, proteins, glycosides, carbohydrates etc. Phytochemical analysis intended to serve as a major resource for information on analytical and instrumental methodology in the plant sciences

Keywords: Antioxidants, Mucuna pruriens, Free radicals, Antibacterial activity, Cup plate method, Disc plate method, Phytochemical