Abstract

ANTIMICROBIAL ACTIVITY OF GRAPE SEED EXTRACT

Ranjitha C.Y, Priyanka S, Deepika R, Smitha Rani G.P, Sahana J,Prashith Kekuda T.R*

ABSTRACT

Grape pomace (skin and seed) is produced during winemaking and is considered to be a waste product of wine industries. Grape seed is known to exhibit various bioactivities. In the present study, we determined antimicrobial activity of grape seed extract (GSE). Agar well diffusion assay was conducted to determine inhibitory effect of GSE against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infection. S. aureus and E. coli was inhibited to high and least extent respectively by GSE. Poisoned food technique was performed to screen antifungal effect of GSE against Colletotrichum capsici. GSE was effective against mycelia growth of C. capsici (>65% inhibition). GSE can be used in the treatment of urinary tract infections and in the management of anthracnose of chilli.

Keywords: Grape seed extract, Agar well diffusion, Urinary tract infection, Poisoned food technique, Colletotrichum capsici.