Abstract

PRODUCTION OF STREPTOMYCIN FROM STREPTOMYCES GRISEUS UNDER SOLID STATE FERMENTATION, & ITS PRODUCTION ENHANCEMENT BY MUTATION AND ANALYSIS BY HPLC

Mamatha J*, Sudipa Bhadra, and Mahesh M

ABSTRACT

The actinomycete strain Streptomyces griseus isolated from soil is used for the production of streptomycin, a potent antibiotic drug which is of great commercial importance. The strain was identified by morphological features and several biochemical tests. Further they were grown as a pure culture in starch casein agar media. Mutation was done both by physical and chemical methods and it was found that the antimicrobial activity was increased by 10% for the mutated strain kept under UV light for 5mins. Production was achieved by solid state fermentation using oranges, pineapple and sugarcane bagasse, as the substrate which are cheaply available. Purification was done by filtration with charcoal and acidified methanol followed by evaporation to produce antibiotic in powder form. High purity was obtained during analysis by High Pressure Liquid Chromatography (HPLC) and it was shown that the production increased by 61.97% with oranges and 4.88% increase with pineapple while there was an 11.64% increase in production with sugarcane bagasse using the physically mutated sample. Hence it was concluded that mutation with UV light for 5mins and solid state fermentation using oranges as substrate yielded the best results. This paper also reviews the possible methods of increasing the production, efficiency and cost effectiveness of streptomycin at the industrial scale.

Keywords: Streptomyces griseus, Mutation, antimicrobial activity, solid state fermentation, High Pressure Liquid Chromatography (HPLC).