Abstract

ARE RENALASE RS2576178 AND RS10887800 POLYMORPHISMS ASSOCIATED WITH PREGNANCY INDUCED HYPERTENSION?

Khaled A. Elsetohy, Mohamed A. S. Al-Ghussein*, Dina Sabry Adel M. Nada, Ashraf A. Eldaly and Amr H. Wahba

ABSTRACT

Background: Pregnancy induced hypertension (PIH) etiology was uncertain. Even if several explanations were suggested, the relation between renalase gene polymorphism and hypertension via renalase catecholamines catabolism activities may be an etiology of PIH that we aimed to investigate. Methods: Known etiological hypertension renalase rs2576178 and rs10887800 polymorphisms were selected. Egyptian 52 PIH patients (study group) were enrolled and compared with 50 normotensive pregnant women (control group). RFLP-PCR was used to assess renalase polymorphism. For our knowledge it is the first time that systolic and diastolic BP was measured for each genotype and allele separately. Results: Genotypes distribution of rs2576178 were significantly different in PIH patients that constitute 48% GG as dominate risk factor in comparison to 60% AA as dominate protective factor in normotensive subjects. Combined with SNP polymorphisms, alleles’ frequency was conversed; A allele 72% dominated in nomotensive and G allele 67.3% dominated in PIH patients. Similarly, rs10887800 genotypes distribution were significantly different in PIH patients that constitute 63.5% AG as dominate risk factor in comparison to 58% AA as dominate protective factor in normotensive subjects. Correspondingly, SNP polymorphisms were associated with alleles’ frequency conversion A allele 73% dominate in nomotensive and G allele 66.3% dominate frequency in PIH patients. Systolic and diastolic BP significantly increased when compared study and control groups results. Fascinatingly, systolic and diastolic BP values were graduated according the genotypes and alleles dominance. Conclusions: Not only, renalase GG rs2576178 and AG rs10887800 polymorphisms are associated with PIH etiology, but also they can be assessed as PIH predictor.

Keywords: Renalase, rs2576178, rs10887800, genotypes, alleles, pregnancy induced hypertension.