Abstract

IN VITROANTIMICROBIAL ACTIVITY OF GARLIC,ONION, GARLIC-ONION COMBINATION (AQUATIC ANDOIL) EXTRACT ON SOME MICROBIAL PATHOGENS IN BABYLON PROVINCE, IRAQ.

Hiba Jassim Hamza 

ABSTRACT

Background: Medicinal plants Garlic and Onion(Allium sativumand Alliumcepa)respectively,can be described as those plants in which one or more of its organs contain substances that can be used for chemotherapeutic purposes or precursors for the synthesis of useful drug.The use of plants in treating ailments has been in practice since a long time ago. Aims: The study aims at determining the antibacterial activity of aquatic garlic,onion and garlic-onion combination extract,then garlic oil,onion oiland garlic-onion oil combination. Methods: The antibacterial effects of aquatic garlic,onion and garliconion combination extractthen garlic oil,onion oiland garlic-onion oil combinationagainst five Gram-positive and eight Gram-negative bacterial & one yeast isolates including Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus viridans, Streptococcus pneumoniae, Pseudomonas aeruginosa, Enterobacteraerugenes, Klebsiellapneumoniae, Escherichia coli, Salmonella typhi, Proteus mirabilis, Serratiamarcescans , Acinetobacterbaumanni,and Candida albicans(fungi)all of them were studied. Results: Antibacterial activity of these aqueous & oil extracts by welldiffusion method were characterized by inhibition zones of five Gram-positive,eightGramnegativeand one yeast pathogenic microbes. All organisms tested were highly sensitive to garlic-onion combination (aqueous and oil extract), then garlic (aqueous and oil extract), whereas all organisms tested were slightly sensitive to onion(aqueous and oil extract). Conclusion: Natural spices of Garlic and Onion (aqueous and oil extract) possess antimicrobial activity against our natural enemies like bacterial pathogens and further evaluation is necessary

Keywords: Garlic, Onion, Garlic-Onion Extract combination,essential oil, antibacterial activity.