Abstract

USING RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION TO EVALUATE GENOTOXICITY OF KBRO3 AND TREATMENT WITH CAPE GOOSEBERRY

Rokaya H. Shalaby, Ibrahim A. Ibrahim, Thanaa M. Badr*

ABSTRACT

Cape gooseberry (Cg) has been an abundant source of highly effective phytochemicals which offer great potential in the fight against cancer. The goal of the present study to investigate genotoxicity of potassium bromate (KBrO3) in mice and treatment with Cg and interferon-α. Ninety male Swiss albino mice were divided into six groups, 15 animals for each group. Mice were received KBrO3, Cg orally and interferon-α subcutaneously. Molecular changes were detected using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). The most important changes after using primer 5`AGGGGTCTTG3` that was quantitative mutation 3 times between control mice and mice treated with KBrO3 at RF 0.68. Also, one common band appeared in control group and all treated groups at RF 0.68 with M.wt. 300 bps. In addition, similarity index (S.I.) between control mice and mice treated with 1g/kg Cg and interferon together is one as compared to mice treated with KBrO3 (S.I.= 0.50). Using primer 5`GAAACGGGTG3` the similarity index between control mice and mice treated with 500mg/kg Cg was 0.80 as compared to mice treated with KBrO3 (S.I.= 0.00). Using six different primers showed that potassium bromate produced complete and partial disturbance in the DNA sequence (S.I.= 0.0 or 0.67). In addition, treatment with Cg decreased alterations occurred in the DNA sequence which was complementary to primers as compared to mice treated with potassium bromate alone. Treatment with interferon-α and 1g/kg Cg together reduced damage to DNA more than treatment with interferon-α alone. From these findings it is to be suggested that Cape gooseberry as new source of bioactive phytochemicals and functional foods.

Keywords: Potassium bromate; Cape gooseberry; random amplified polymorphic DNA polymerase chain reaction and interferon-?.