Abstract

INVITRO ANTIOXIDANT POTENTIAL OF Phasealous lunatus L. SEEDS

Marimuthu Krishnaveni,* Nagaraj Nandhini., Ravi Dhanalakshmi

ABSTRACT

The key attribute of an antioxidant is its ability to trap free radicals. Various methods have been used to observe and evaluate the antioxidant activity of foods. The assessment of secondary metabolites such as phenolics, flavonoids and antioxidant activity by various methods was carried out with aqueous extract of dry Christmas lima bean seeds. The concentrations used for the study are 75, 100mg. Among the secondary metabolites tested, the total flavonoid content was found to be high when compared to total phenolics. The total phenolics content present in 100, 75mg of dry christmas lima bean seeds was found to be 28.66±1.52, 19.53±6.11mg/g whereas, the flavonoid content observed was 54.0±2.0, 33.73±0.75. Among the antioxidant activities studied using different methods, the nitric oxide scavenging activity(30.33±2.51mg/g), reducing power activity (35.0±2.0mg/g) as well as total antioxidant activity(28.66±1.52mg/g) was found to be good with dry Christmas lima bean seed powder whereas, the activity was reduced with metal chelating activity (21.0±1.32 mg/g). Likewise, among the concentrations used 100mg showed very good antioxidant potential. The results obtained showed increasing antioxidant activity with increasing concentration.

Keywords: Antioxidants, Dry Christmas lima bean seeds, Flavonoids, Phenolics, Phasealous lunatus.