Abstract

HIBISCUS SABDARIFFA L. VAR. AMV1 : MUTAGENIC EFFECTIVENESS AND MUTAGENIC EFFICIENCIES BY CHEMICAL MUTAGEN ETHYL METHANE SULPHONATE AND N-METHYL-NNITRO N- NITROSOGUANIDINE

Padma Shrivastava* and Chhota Raja

ABSTRACT

Different types of mutations may even be produced experimentally by mutation inducing agents or “mutagens” and the type of mutation is called experimental or artificial or induced mutations. Efficient mutagenesis is the induction of desirable changes free from the usually associated undesirable effects. Mutagenic efficiency of a treatment induced undesirable effects, in this work the EMS and NMG were used in Hibiscus sabdariffa L. Var. AMV1 for Mutagenic Effectiveness and Mutagenic Efficiencies. The different concentration of EMS and NMG was used to treat the dry and pre-soaked seeds (6,12,18 hrs). The M2 population was screened for morphological mutations till the maturity of plants. The frequency of induced mutations was calculated per 100 M2 seedling bases.

Keywords: Ethyl methane Sulphonate (EMS), N-methyl-N-nitro N- nitrosoguanidine(NMG), Mutagenic Effectiveness, Mutagenic Efficiency, Pre-soaked seeds.