Abstract

MECHANISM OF ACTION OF THE COMPOUNDS FROM TERMINALIA CHEBULA AND ADATHODA VASICA TARGETED AGAINST URINARY TRACT INFECTION

*Jasmine R, Aishwarya, Selvakumar BN

ABSTRACT

Among the several infections that target women, urinary tract infections are more prevalent. Antibiotics administered cause a lot of side effects. The current study was designed to evaluate the traditional claims of the two Indian medicinal plants for treating urinary tract infection. The traditional claims were investigated by using ethanol and acetone extracts of the plants against the isolated uropathogens and to study their role as inhibitors to bacterial adhesion of urinary tract. Antibacterial activity was determined by the disc diffusion assay and the Minimal Inhibitory Concentration was evaluated by micro broth dilution method. The active compounds isolated (already reported) were investigated for their action against bacterial adhesion in urinary tract by docking. The ethanol and acetone extracts of the two chosen plants were used for the study possessed significant antibacterial activity, whose zones ranged from 10 to 22mm diameter. The minimum inhibitory concentration (MIC) of the plants fell between 62.5 and 250 μg/ml. The active compounds isolated were investigated for their action against bacterial adhesion in urinary tract. The docking results indicate that both the plants inhibit the growth of the chosen bacteria to an appreciable degree. When the activity of the compounds were analyzed, they were found to obey all the 5 rules of ADME, hence, can be used as efficient drugs and could be used as possible alternatives to antibiotics for treating UTI.

Keywords: Urinary tract infection, antibacterial, MIC, Adathoda vasica, Terminalia chebula.