Abstract

ANTIBIOGRAM TYPING AND ERIC-PCR GENOTYPING OF ESCHERICHIA COLI ISOLATED FROM DIFFERENT CLINICAL SAMPLES

Mun,im R. Ali .; Shaymaa Khudhr Al–Alak .;Ashraf Sami Hassan.; IstabraqMohammed Atia

ABSTRACT

Escherichia coli is a common cause of infection affecting both male andfemale individuals. Maximum E. coli isolates were recovered from urine (59) followed by ear discharge (5), stool and vaginal swap (3 for each). The mean age of infected patients was range (less1 to 10 year) and male: female ratio was 5:15. While the less infected age was range (80-90 year). Dendrogram analysis of antibiotic used has been able to show a very higher resolving strain differentiation. The antibiogram patterns of the 70 isolates of E.coli of differnt origin were determined and among them 2 multiple resistance patterns A &B were observed, among A group the isolates clusters in to two sub group ,while ERICPCR dendrogram showing the genetic diversity/relatedness of the 44 Escherichia coli strains. All 90 isolates were tested by ERIC-PCR, from which 26 isolates 37.14% were untypeble, while only 44 isolates were successfully amplified by ERIC-PCR. ERIC-PCR of E. coli isolates generated multiband fingerprint patterns. ERIC-PCR yielded bands between 200bp to 1200 bp ranging from 1 to 5 bands in an individual sample. the results indicated that both methods were able to discriminate epidemiological clusters, with only a few discrepancies. The results of this study underscore the antibiogram typing is a simple, time consuming, with the cost of a few method, which researchers were able to investigate of E. coli epidemic when advanced molecular typing methods are not available.

Keywords: ERIC-PCR , E. coli , Dendrogram , antibiogram.