Abstract

PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF Blepharis maderaspatensis (L.) B.Heyne ex Roth LEAF

Suriyavathana Muthukrishnan*, Indupriya Santhanam and Subha Palanisamy

ABSTRACT

Objectives: To evaluate the phytochemical screening and enzymatic and non-enzymatic antioxidants activity of the leaf extract of Blepharis maderaspatensis. Methods: Phytochemical screening of the leaf extract was done to determine the phytochemical constituents in the various solvents. The enzymatic antioxidants (Superoxide dismutase, Catalase, Glutathione-s-transferase and Glutathione peroxidase) and non-enzymatic antioxidants ( Reduced glutathione, Vitamin C and Vitamin E) were estimated. Results: The phytochemical analysis comprehensively validates that the leaf extract of B.maderaspatensis possessed therapeutically important secondary metabolites such as Alkaloids, Phenols, Flavanoids, Steroids, Saponins, and Tannins. The levels of enzymatic and non- enzymatic antioxidants in the leaf extract of B.maderaspatensis shown significant scavenging activity which emphasis its antioxidant status. Conclusion: The results of the present study revealed that the leaf extract of Blepharis maderaspatensis has exhibited a broad spectrum of antioxidant activities and could be serve as an excellent source of natural antioxidants.

Keywords: Blepharis maderaspatensis (L.) B.Heyne ex Roth, Phytochemical, Enzymatic antioxidants, Non-enzymatic antioxidants