EVALUATION OF ANTICANCER ACTIVITY OF METHANOLIC EXTRACT OF CARALLUMA FIMBRIATA WALL. AGAINST LUNG CANCER CELL LINE
Priya D*, Rajaram K*, Sureshkumar P
ABSTRACT
The present study is designed to evaluate the in-vitro anticancer
activity of methanolic extract of Caralluma fimbriata Wall. against
Lung cancer cell line A-549 for its effects on cell viability, growth
inhibition and cell morphology. Cell viability and inhibition was
determined by cytotoxicity assay and MTT assay. Cell morphology
was observed under inverted microscope. The cytotoxicity effect of
Methanol extract carried out using various concentration in L6 muscle
cells. The result showed that the methanol extract upto 100μg/ml
having lesser toxicity. Hence the doses for antiproliferative activity
fixed within that concentration. The antiproliferative results showed
decreased cell viability and increased growth inhibition in a dose and duration dependent
manner and also altered cell morphology after treatment with methanolic extract of
Caralluma fimbriata Wall. The data demonstrated that methanolic extracts of Caralluma
fimbriata Wall. has a potential anticancer agent on A-549.
Keywords: Caralluma fimbriata Wall., Anticancer, cytotoxic, 3-(4,5-dimethylthiazol-2-yl)- 2,5- diphenyltetrazolium bromide (MTT) assay, A-549 and L6 cell line.
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