IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF WRIGHTIA TINCTORIA LEAVES
Jyotiram A. Sawale*, C.V. Panchal , Suhas Padmane, B N Poul and J R Patel
ABSTRACT
Background: Since free radicals play such an important role in the
disease scenario of an individual, a thorough understanding of the
various physiologically significant free radicals is of paramount
importance before the search of the radical scavengers or the
antioxidant principles to treat the physiological disorders caused by
them such as inflammation. Objective: To explore newer anti-oxidant
and anti-inflammatory agent from natural sources. Material and
Methods: The antioxidant activity of the methanolic extract of
Wrightia tinctoria leaves was determined by measuring its 1,1
diphenyl-2-pycryl hydrazyl (DPPH) radical scavenging activity, reducing power and ability
to inhibit lipid peroxidation. Anti-inflammatory activity was evaluated by using carrageenan
induced rat paw oedema model in healthy wistar albino rats. Results and Discussion:
Methanol extract exhibited strong total antioxidant activity. Concentrations of 50, 100, 200,
400, 800 and 1000μg /mL showed 52.34, 53.69, 53.69, 56.37, 58.60 and 61.07% inhibition of
lipid peroxidtion respectively. In addition methanol extract had effective reducing power,
DPPH free radical scavenging activity at 400μg/mL, the same extract was also evaluated for
its anti-inflammatory activity at doses 100, 200 and 400mg/kg.Conclusion: Methanolic
extract showed predominantly significant activity in a dose dependent manner, which is
comparable to reference standard diclofenac sodium. Phytochemical studies indicated
presence of alkaloids and phenols are the major components in the extract and it may be
further concluded that these constituents may be responsible for antioxidant and antiinflammatory
activity.
Keywords: Antioxidant activity, Wrightia tinctoria, DPPH, Lipid peroxidation, reducing power
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