Abstract

SCREENING OF SOME PHYTOPATHOGENIC FUNGI FOR THEIR ANTIMICROBIAL POTENTIAL

Shyamji Shukla*, Harshita Shukla and Akhilesh Kumar Pandey

ABSTRACT

Plant Pathogenic fungi incite a majority of known plant diseases by virtue of their potential for production of some toxic secondary metabolites. The metabolites, synthesised by phytopathogen to establish them self as pathogen, however, have not been screened for their ability to be used as new antimicrobial drugs. So plant pathogenic fungi are poorly investigated groups of microorganisms that represent an abundant and dependable source of bioactive and chemically novel compounds with potential for exploitation in a wide variety of medical, agricultural and industrial areas. Therefore during the course of the present study, screening of 80 fungi recovered from different habitats of Madhya Pradesh was done to select potential antimicrobial producing strain. The general screening methods such as diffusion assays and dilution assays were used for screening antimicrobial activity. In the present investigation screening of some phytopathogenic fungi was done for their antimicrobial potential. Maximum broad spectrum activity was recorded in case of cell free culture filtrates obtained from the fermented medium of Fusarium roseum FGCC#61 against the test organisms so it was selected for further study. The antimicrobial moiety was then isolated by using a solvent extraction method. The active principle resided in ethyl acetate extracted fraction of the CFCF (Cell Free Culture Filtrate).

Keywords: Phytopathogenic fungi, Antimicrobial activity, CFCF (Cell Free Culture Filtrate), Bioassays.