BACTERICIDAL EFFECT OF AEGLE MARMELOS EXTRACTS ON PATHOGENIC BACTERIA
R. K. Sujatha and *S. Rajan
ABSTRACT
Pathogenic microorganisms are effectively controlled by making use of
modern combined antibiotics. Improper and overuse of antibiotics
cause various side effects and creates resistance among bacterial
pathogens. To overcome these problems peoples turned towards the
use of medicinal plants as therapeutic agents. Aegle marmelos fruit
pulp is an efficient traditional phytomedicine used for the treatment of
various infections. Antibacterial activity of aqueous and ethanolic
extract of Aegle marmelos fruit pulp s were determined using disc
diffusion, modified agar dilution methods. MIC, MBC, Percentage of
inhibition and IC50were calculated using modified drug dilution
method. The results showed that the fruit pulp extracts of A. marmelos
have antimicrobial activity against E. coli, Salmonella, Shigella, Klebsiella, Pseudomonas,
Staphylococcus aureus, Streptococcus pyogenes. Ethanolic extracts showed the highest
inhibition zone of 16.0±1.0mm diameter against Pseudomonas aeruginosa. The plant extracts
were shown to have a best MIC at 150±050μg/ml for ethanolic extract and 216.6±028.8
μg/ml for aqueous extract. Percentage of inhibition created by the extracts against the
pathogens varied from 47.6% to 92. 6% 2ith best activity against Escherichia coli by both the
extracts. Effective IC50 exhibited by ethanolic extract 112.9 μg/ml concentrations against E.
coli.
Keywords: Bacterial pathogens, MIC, MBC, Antibacterial activity, IC50, Percentage inhibition, Aegle marmelos, Fruit pulp.
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