ACUTE EFFECTS OF GEMCITABINE ON SEMINIFEROUS TUBULAR HISTOMORPHOMETRY IN MICE MODEL
Viveka. S*, Ajay Udyavar, Balakrishna Shetty, Santhosh Kuriakose, Sudha. M. J.
ABSTRACT
Chemotherapeutic agents have adverse effects on spermatogenesis.
The gonadotoxic effects of these agents are of significance in young
cancer patients undergoing chemptherapy. Gemcitabine is a novel
antimetabolic anticancer drug used frequently in treatment of many
cancers. This study was designed to evaluate the acute effects of
gemcitabine on spermatogenesis in mice model. Gemcitabine in high
and low doses (80 and 40 mg kg-1) injected intraperitoneally to inbred
Swiss albino mice. Gross testicular features and seminiferous tubular
histomorphometry was studies at the end of 7th, 14th day. Sperm shape
abnormalities studied. We noted that seminiferous tubular morphology
was altered significantly, showing reduction in height, perimeter and
area in a dose dependent manner. Sertoli cell number decreased.
Basement membrane had reduced thickness. Intertubular spaces has
reduced. Sperms had abnormal shapes with banana heading, decapitation. It was concluded
that gemcitabine affects the process of spermatogenesis adversely in a dose dependable
manner.
Keywords: Swiss albino mice, spermatogenesis, Gemcitabine, seminiferous tubules, Sertoli cells
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