Abstract

SYNTHESIS AND CHARACTERIZATION OF LAYER BY LAYER MAGNETIC NANOPARTICLES OF METHOTREXATE AND MELPHALAN

Subbarayan Shanmugavel*, Venkatachalam Karthikeyan

ABSTRACT

Synthesize of polystyrene nanoparticle by using Pickering emulsion Polymerization method without using surfactant. Designing of a magnetic nano core /shell assembly by using polystyrene nanoparticles as templates in which chitosan polymer as shell material and a hydrophilic anticancer drug (Methotrexate and Melphalan) are loaded into the core. Surface coating of the designed core/shell assembly with polyelectrolyte (sodium alginate). Step by step characterization in the experiment to conform the chemical reactions involved in each step. The size of the formulations was found to be 250-300nm. Surface morphology was analyzed by scanning electron microscope and it was spherical in nature. Magnetic property was evaluated by vibrating sample magnetometer which gives a hysteresis loop with magnetization value 50emu/g indicates that the super paramagnetic nature of the formulation. Tumour targeting is possible by the synthesized magnetic nanocarrier by means of using magnetic field to carry the nanocarrier towards the tumor site. Targeted and controlled drug delivery for combination (three in one) of drugs is being possible by the formulated layer- bylayer magnetic nanoparticles, it may have great potential in chemotherapy of cancer.

Keywords: Methotrexate; Melphalan; Magnetic nanoparticles; Chitosan; Sodium alginate; drug targeting.