PHYTOCHEMICAL SCREENING OF STEROLS EXTRACTED FROM CICHORIUM INTYBUS IN VIVO AND IN VITRO
Meena Agarwal*, Renu Sarin
ABSTRACT
Context: Natural habitats for medicinal plant are disappearing fast and
together With environmental instabilities, it is increasingly difficult to
acquire plant derived compounds. This has prompted scientists to
consider the possibilities of using cell cultures as an alternative
supply for the production of plant natural products. Aim: The present
investigation deals with the establishment of callus cultures from nodal
explants of Cichorium intybus, to extract sterols from the raised callus
and plant parts. Materials and Methods: Callus culture of Cichorium
intybus has been established on Murashige and Skoog’s medium
supplemented with BAP using nodal explants. Growth index (GI) was
calculated for the tissues obtained after 2, 4, 6 and 8 weeks,
respectively. Calli of 2, 4, 6 and 8 weeks age and plant parts were used
for extraction of sterols using standard method. Results: GI was found maximum in 6 weeks
old tissue (5.12).Stigmasterol and β-sitosterol was identified from 6 week old callus and plant
parts through, thin layer chromatography (TLC), preparative TLC, Melting point and Infrared
spectral and GC-MS studies. Content of Stigmasterol and β-sitosterol in 6 week old calli and
plant parts was also estimated using standard Spectrophotometric method. Conclusion: The
protocol of callus induction of C.intybus opens new vistas that could facilitate phytochemical
production, extraction of pharmaceuticals and their screening against different human
pathogens from the callus without harvesting the plant itself.
Keywords: ?-sitosterol , GC-MS ,Growth indices, stigmasterol, Tissue culture,TLC.
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