Abstract

COMPARISON OF GREEN AND BLACK TEA ANTIOXIDANT ACTIVITY WITH LIPPIA CITRIODORA

Samira Ildashti , *Ali Mirzaei , Ali Akbar Abulfathei

ABSTRACT

Introduction and Aim: Tea, Camellia sinensis family Theaceae, is the second beverage consumed daily in the world for centuries.Lippia citriodora (Verbenaceae) is a medicinal plant that mostly used as a spice with different biological activity which grows in South America and North Africa and Southern Europe. The aim of recent study was managed to compare the antioxidant properties and phenolic contents of 2 commercial green and 4 black tea brands in Iran with Lippia. citriodora as a new beverage. Materials and Method: The samples (2 g) were infused in 1000 ml of boiling water in a flask for 30 Min. After extraction, the infusions were filtered through a filter paper No. 1.Total phenolic and flavonoids content was measured according to the Folinciocalteus and aluminum chloride methods .respectively. Total antioxidant activity were measured by2,2-diphenyl-1-picrylhydrazyl radical DPPH method .Statistics analysis between different groups was accomplished by analysis of variance using the ANOVA and followed by post hoc tests. Results: Green tea infusions and Lippia citriodora had the highest antioxidant activity (50.3-54%), total phenol (12-16 g % GAE) and flavonoids (8.5-9.8 mg Rutin %) contents .There was a positive correlation between antioxidant activity and total flavonoid content. Conclusion: Tea infusions and Lippia citriodora leaves extract were demonstrated potent of antioxidant activity and high level phenolic compounds. Lippia citriodora infusion with high flavonoid content and antioxidant activity has been comparable with Iranian commercial brands tea in Yasuj Iran.

Keywords: Antioxidant activity, DPPH, total phenols, Flavonoids, Camellia sinensis , Lippia citriodora.