Abstract

PROTECTIVE EFFECT OF PISTACIA KHINJUK ON GENTAMICININDUCED NEPHROTOXICITY IN RATS

Tayyebeh Ghaedi, *Ali Mirzaei , Behzad Laameerad

ABSTRACT

Introduction and Aim: Gentamicin (GM) is used for treatment of urinary tract infections which induce nephrotoxicity. This study designed to investigate the possible protective effects of Pistacia khinjuk against GM-induced renal damage in rats. Materials and Methods: The ethanol extract of Pistacia khinjuk fruit was administered orally once for 14 days. Male wistar rats were divided into five groups of six rats in each one; first group served as control and received normal saline, second group was received GM (80 mg/kg/d, intraperitoneally) for 14 days, third , fourth and sixth groups were received simultaneous GM plus extract 150, 300 and 600 mg / ml respectively. The rats were sacrificed on the 15th day and serum was collected. Nephroprotective potential was estimated by renal functional tests such as blood urea nitrogen (BUN), creatinine(CR), total protein(TP) , sodium (Na) ,potassium(K) levels and kidneys were dissected out for determination of reduced glutathione (GSH) and malondialdehyde (MDA) levels in renal tissues homogenate. Results: The animals which received only GM causes a significant increase in levels of CR and BUN, MDA, and significantly decrease in TP and GSH concentrations when compared with the control group. Treatment with Pistacia khinjuk produced amelioration in biochemical indices in plasma and renal tissue when compared with the GM treated group. Conclusion: simultaneous treatment of Pistacia khinjuk might have produced amelioration in biochemical indices against GMinduced nephrotoxicity. It was concluded that Pistacia khinjuk is an effective nephroprotective agent as a novel natural antioxidant.

Keywords: Gentamicin, Pistacia khinjuk ; nephroprotective, BUN, MDA.