Abstract

ANTIHEPATOTOXIC AND ANTIOXIDANT ACTIVITY OF NARDOSTACHYS JATAMANSI AGAINST D-GALACTOSAMINEINDUCED HEPATOTOXICITY IN EXPERIMENTAL ANIMALS

Ramesh Kumar Gupta*, Rajnish Kumar Singh1, Vaishali, Susanta Kumar Panda,Padala Narasimha Murthy, Ghanashyam Panigrahi, Sudhansu Ranjan Swain, Jagannath Sahoo

ABSTRACT

Background: Liver diseases remain one of the serious health problems. Natural treatments from medicinal plants are considered to be effective and safe medicaments for hepatotoxicity. Information collected from ethnic groups has played an important role in the discovery of new products from plants as hepatoprotective agents. Aim: The purpose of the present study was to assess the hepatoprotective and antioxidant effect of Nardostachys Jatamansi extract against D-galactosamine drug-induced liver toxicity in experimental animals. Method: 50% Ethanolic extract of rhizome of Nardostachys Jatamansi (NJE, 250 and 500 mg/kg body weight) was evaluated using experimental models; D-galactosamine (D-GalN) (200 mg/kg, body weight, i.p.) induced hepatotoxicity in experimental animals. Result: The hepatoprotective activity was assessed using various biochemical parameters like aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatise (ALP), total bilirubin (TBL), albumin (ALB), total protein (TP), lactate dehydroginase (LDH), and serum cholesterol (CHL). Meanwhile, in-vivo antioxidant activities as lipid peroxidation (LPO), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were measured in rat liver homogenate along with ATPase and GWORLD 6-Pase was also evaluated. Obtained results demonstrated that the treatment with NJE significantly (P<0.05-P<0.001) and dose-dependently prevented drug induced increase in serum levels of hepatic enzymes. Furthermore, NJE significantly (up to P<0.001) reduced the lipid peroxidation in the liver tissue and restored activities of defence antioxidant enzymes GSH, SOD, CAT, ATPase and G-6-Pase towards normal levels. Conclusion: The results of this study strongly indicate the protective effect of NJE against liver injury which may be attributed to its hepatoprotective activity, and there by scientifically support its traditional use.

Keywords: Nardostachys Jatamansi, D-galactosamine, Alkaline phosphatise, Albumin, Catalase.