Abstract

NITRIC OXIDE AND CIPROFLOXACIN AGAINST OXIDATIVE STRESS INDUCED BY SALMONELLA TYPHIMURIUM

*S.S.Haque

ABSTRACT

Background: Glutathione reductase (GR) is an important antioxidant enzyme that reduces glutathione disulfide (GSSG) to the sulfhydryl form GSH by the NADPH-dependent mechanism. GR belongs to the defense system protecting the organism against chemical and oxidative stress. Oxidative stress due to overproduction of reactive oxygen species and impairment in antioxidant defense mechanisms have been suggested as possible factors contributing to the pathogenesis of typhoid. Nitric oxide is a gaseous free radical produced in the body by the isoenzyme nitric oxide synthase (NOS) using L-arginine as a substrate. Materials and Methods: Glutathione reductase activity was measured as described by Calberg and Mannervic. Results and Discussion: Infection of mice with bacteria resulted in decrease in the GR activity by 6.06%, 7.87% and 8.81% at day 8, 11 and 14 as compared to saline treated control and increase activity of glutathione reductase was noted in formulated drugs treated groups.

Keywords: Nitric oxide, Glutathione Reductase, Typhoid.