EFFECT OF LEAF AND ROOT EXTRACTS FROM THOTTEA SILIQUOSA (LAM.) DING HOU. A MEDICINAL PLANT ON ANTIOXIDANT STATUS
Saiba A.N, Remya Krishnan and Murugan K.*
ABSTRACT
Experimental evidence proves that most of the plants possess a wide
array of biological and pharmacological potentialities that may protect
tissues from oxidative damages. The aim of this study was to screen
various solvent extracts of root and leaves of Thottea siliquosa to
evaluate potent antioxidant activity in vitro, total phenol, alkaloid,
saponins and flavonoid contents in order to find possible sources for
future novel antioxidants for pharmaceutical formulations. The
methodologies employed are analytical and biochemical. Thorough
study was performed with the petroleum ether and ethyl acetate
extracts of root and leaf of the plant by in vitro chemical analysis and
antioxidant potentialities. Preliminary qualitative analysis revealed the
presence of alkaloids, tannins, glycosides, coumarins, flavonoids and
saponinss. Phenols (2.5 -4.8 mg/g), alkaloids (43-287 μg/g) and saponins (66-303 μg/g)
represented the highest level both in the petroleum ether and ethyl acetate extracts of root and
leaves. The antioxidant potentialities in terms of DPPH radical, hydroxyl radical, superoxide
anion and nitric oxide quenching capacity revealed varying responses. The IC50 values are
comparable with the standards such as ascorbate and butylhydroxy toluene. Lipid
peroxidation level was comparatively inhibited by the extracts. Results from present study
suggest that the phytochemicals in the extracts of T.siliquosa act as antioxidant agents as it
was substantiated by its free radical scavenging activities.
Keywords: Thottea siliquosa (Lam.) Ding Hou., Antioxidant potentiality, DPPH Radicals, Superoxide anion, hydroxyl radicals.
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