Abstract

IN VITRO ANTI-INFLAMMATORY ACTIVITY OF CARMONA RETUSA (VAHL.)

Chandrappa CP¹, Govindappa M¹*, Anil Kumar NV² and Channabasava R¹

¹Department of Biotechnology, Shridevi Institute of Engineering and Technology, Tumkur572106, Karnataka, India.

²Department of Chemistry, Manipal Institute of Technology, Manipal University, Karnataka,India.

ABSTRACT

The anti-inflammatory activity of ethanol extract of stem of Carmona retusa was investigated for in-vitro anti-inflammatory activity by human red blood cell membrane stabilization method, heat induced hemolysis and proteinase inhibitory activity. The anti-inflammatory activity of Carmona retusa found maximum in human red blood cell membrane stabilization method, heat induced hemolysis and proteinase inhibitory activity as 55.72%, 56.37% and 61.75% respectively at 400μg/ml concentration. Minimum anti-inflammatory activity has shown at 50μg/ml concentration as 28.92μg/ml, 15.43μg/ml and 11.33μg/ml in the methods used. IC50 values were calculated for all the methods and found to 30.31, 19.62 and14.53μg/ml for human red blood cell membrane stabilization method, heat induced hemolysis and proteinase inhibitory activity respectively. The anti-inflammatory activity of the sample was comparable with that of the standard diclofenac. The results obtained in the present study suggest that Carmona retusa can be a potential source of antiinflammatory agents.

Keywords: Carmona retusa, anti-inflammatory activity, HRBC, IC50.