Abstract

NATURALLY OCCURRING ACTIVE CONSTITUENT CAN BE ONE OF THE CHOICE TO TREAT ACUTE PANCREATITIS

Sandeep Biradar*

*Jagdishprasad Jhabarmal Tibrewala University, Jhunjhunu, Rajasthan, India.

ABSTRACT

Objective: The present study determined ameliorating effect of limonene on l-arginine induced acute pancreatitis model. Materials and methods: Acute pancreatitis was induced in five groups of rats (n = 6) by L-arginine (2 × 2.5 g/kg, intraperitoneal, 1 h apart) and 1 h later, they received a single oral dose of limonene (100 and 200 mg/kg), methylprednisolone (30 mg/kg) and vehicle (3% Tween 80). A saline (0.9% NaCl) treated group served as a normal control. The effectiveness of limonene was determined at 24 h by analyzing the level of lipase, amylase and proinflammatory cytokines including tumor necrosis factor (TNF)- α interleukin (IL)-6, C-reactive protein (CRP), lipid peroxidation (thiobarbituric acid reactive substances (TBARS), pancreatic myeloperoxidase (MPO) activity, nitrate/nitrite levels, glutathione (GSH), malonyl dialdehyde (MDA and SODsuperoxide dismutase Results: It was noted that limonene and methylprednisolone treatments significantly (P < 0.05) ameliorate the L-arginine-induced increases in pancreatic wet weight/body weight ratio and decreased the serum levels of amylase and lipase, and TNF-α, IL-6 and CRP as compared to the vehicle control. Also, pancreatic levels of MPO activity, TBARS, and nitrate/nitrite were significantly lower. Histological findings confirmed the amelioration of pancreatic injury by limonene Conclusion: limonene has the potential to heal acute pancreatitis by acting as an antiinflammatory and antioxidant agent.

Keywords: Antioxidant, C-reactive proteins, Limonene, L-arginine, Myeloperoxidase.